Identification of a pathogenic isolate-specific 30,000-Mr antigen of Entamoeba histolytica by using a monoclonal antibody.

TitleIdentification of a pathogenic isolate-specific 30,000-Mr antigen of Entamoeba histolytica by using a monoclonal antibody.
Publication TypeJournal Article
Year of Publication1990
AuthorsTachibana, H, Kobayashi, S, Kato, Y, Nagakura, K, Kaneda, Y, Takeuchi, T
JournalInfect Immun
Volume58
Issue4
Pagination955-60
Date Published1990 Apr
ISSN Number0019-9567
Call Numbernewpubs20101112_Schane.pdf
KeywordsAnimals, Antibodies, Monoclonal, Antigens, Protozoan, Entamoeba histolytica, Female, Fluorescent Antibody Technique, Mice, Mice, Inbred BALB C, Molecular Weight
Abstract

A monoclonal antibody (MAb) produced against trophozoites of Entamoeba histolytica strain HM-1:IMSS, reacted with all of 42 isolates and 4 clones showing pathogenic zymodeme (Z) patterns, i.e., Z-II, Z-II alpha-, Z-II (glucose phosphate isomerase: gamma +), Z-VII, Z-VII (glucose phosphate isomerase: alpha lack, gamma +), Z-XI, Z-XIV, and Z-XIX, regardless of culture conditions, geographical origins, or host symptoms in an indirect fluorescence antibody test. In contrast, the MAb failed to react with 14 isolates possessing nonpathogenic zymodemes Z-I and Z-VIII and did not react with other enteric protozoan parasites, such as E. histolytica-like Laredo, Entamoeba hartmanni, Entamoeba coli, Endolimax nana, Dientamoeba fragilis, Trichomonas hominis, and Giardia lamblia. Western immunoblotting analysis showed that the molecular weight of the antigenic component recognized by the MAb was exclusively 30,000 in pathogenic isolates of different zymodemes. These results suggest that the 30,000-molecular-weight antigen is a marker of pathogenic isolates and that the indirect fluorescent-antibody test with the MAb is useful for the accurate discrimination of pathogenic amebae.

URLhttps://iai.asm.org/content/58/4/955.long
DOI10.1128/IAI.58.4.955-960.1990
User Guide Notes

http://www.ncbi.nlm.nih.gov/pubmed/2180826?dopt=Abstract

Endnote Keywords

depression/Chronic Disease/Screening/health care outcomes

Endnote ID

23660

Alternate JournalInfect Immun
Citation Key7281
PubMed ID2180826
PubMed Central IDPMC258567