|Title||Cytomegalovirus antibodies in dried blood spots: a minimally invasive method for assessing stress, immune function, and aging.|
|Publication Type||Journal Article|
|Year of Publication||2011|
|Authors||Dowd, JBeam, Aiello, AE, Chyu, L, Huang, Y-Y, McDade, TW|
|Date Published||2011 Jan 13|
BACKGROUND: Cytomegalovirus (CMV) is a prevalent herpesvirus with links to both stress and aging. This paper describes and validates a minimally invasive method for assessing antibodies against CMV in finger stick whole blood spot samples for use as an indirect marker of an aspect of cell-mediated immunity.
RESULTS: Analysis of CMV in dried blood spot samples (DBS) was based on modifications of a commercially available protocol for quantifying CMV antibodies in serum or plasma. The method was evaluated through analysis of precision, reliability, linearity, and correlation between matched serum and DBS samples collected from 75 volunteers. Correlation between DBS and plasma values was linear and high (Pearson correlation R = .96), and precision, reliability, and linearity of the DBS assay were within acceptable ranges.
CONCLUSIONS: The validity of a DBS assay for CMV antibodies will enable its inclusion in population-based surveys and other studies collecting DBS samples in non-clinical settings, increasing scientific understanding of the interaction of social and biological stress and immune function.
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|Alternate Journal||Immun Ageing|
|PubMed Central ID||PMC3031243|
|Grant List||R01 AG040115 / AG / NIA NIH HHS / United States |
R21 NR011181 / NR / NINR NIH HHS / United States
R21 NR011181-02 / NR / NINR NIH HHS / United States
T32 HD007545 / HD / NICHD NIH HHS / United States