Validation of Blood-Based Assays Using Dried Blood Spots for Use in Large Population Studies

TitleValidation of Blood-Based Assays Using Dried Blood Spots for Use in Large Population Studies
Publication TypeJournal Article
Year of Publication2014
AuthorsCrimmins, EM, Kim, JKi, McCreath, H, Faul, JD, Weir, DR, Seeman, TE
JournalBiodemography and Social Biology
Volume60
Issue1
Pagination38 - 48
Date PublishedFeb-01-2014
ISSN Number1948-5565
KeywordsBlood-based assays, Genetics, Meta-analyses, Older Adults, Population Health
Abstract

Assessment of health in large population studies has increasingly incorporated measures of blood-based biomarkers based on the use of dried blood spots (DBS). The validity of DBS assessments made by labs used by large studies is addressed by comparing assay values from DBS collected using conditions similar to those used in the field with values from whole blood samples. The DBS approach generates values that are strongly related to whole blood levels of HbA1c, cystatin C, and C-reactive protein. Assessing lipid levels reliably with DBS appears to be a greater challenge. However, even when DBS values and values from venous blood are highly correlated, they are often on a different scale, and using conventional cutoffs may be misleading.

URLhttp://www.tandfonline.com/doi/abs/10.1080/19485565.2014.901885
DOI10.1080/19485565.2014.901885
Short TitleBiodemography and Social Biology
Citation Key8602
PubMed ID24784986
PubMed Central IDPMC4117354