Validation of blood-based assays using dried blood spots for use in large population studies.

TitleValidation of blood-based assays using dried blood spots for use in large population studies.
Publication TypeJournal Article
Year of Publication2014
AuthorsCrimmins, EM, Kim, JKi, McCreath, H, Faul, J, Weir, DR, Seeman, T
JournalBiodemography Soc Biol
Volume60
Issue1
Pagination38-48
Date Published2014
ISSN Number1948-5573
KeywordsBiomarkers, C-reactive protein, Cholesterol, Cholesterol, HDL, Cystatin C, Dried Blood Spot Testing, Glycated Hemoglobin A, Humans, Middle Aged, Reference Values, Reproducibility of Results
Abstract

Assessment of health in large population studies has increasingly incorporated measures of blood-based biomarkers based on the use of dried blood spots (DBS). The validity of DBS assessments made by labs used by large studies is addressed by comparing assay values from DBS collected using conditions similar to those used in the field with values from whole blood samples. The DBS approach generates values that are strongly related to whole blood levels of HbA1c, cystatin C, and C-reactive protein. Assessing lipid levels reliably with DBS appears to be a greater challenge. However, even when DBS values and values from venous blood are highly correlated, they are often on a different scale, and using conventional cutoffs may be misleading.

URLhttp://www.tandfonline.com/doi/abs/10.1080/19485565.2014.901885
DOI10.1080/19485565.2014.901885
User Guide Notes

http://www.ncbi.nlm.nih.gov/pubmed/24784986?dopt=Abstract
Short TitleBiodemography and Social Biology
Alternate JournalBiodemography Soc Biol
Citation Key8602
PubMed ID24784986
PubMed Central IDPMC4117354
Grant ListP30 AG017265 / AG / NIA NIH HHS / United States
R24 AG037898 / AG / NIA NIH HHS / United States
U01 AG009740 / AG / NIA NIH HHS / United States
P30 AG17265 / AG / NIA NIH HHS / United States